LOCUS Exported 11454 bp ds-DNA linear BCT 04-SEP-2020 DEFINITION Yersinia ruckeri strain YR71 plasmid pYR1, complete sequence. ACCESSION CP000602 VERSION . KEYWORDS . SOURCE Yersinia ruckeri ORGANISM Yersinia ruckeri REFERENCE 1 (bases 1 to 11454) AUTHORS Welch TJ, Fricke WF, McDermott PF, White DG, Rosso ML, Rasko DA, Mammel MK, Eppinger M, Rosovitz MJ, Wagner D, Rahalison L, Leclerc JE, Hinshaw JM, Lindler LE, Cebula TA, Carniel E, Ravel J. TITLE Multiple antimicrobial resistance in plague: an emerging public health risk. JOURNAL PLoS ONE 2007;2:e309 PUBMED 17375195 REFERENCE 2 (bases 1 to 11454) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Sep 4, 2020 from SnapGene 5.1.5 https://www.snapgene.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; date: "2007"; volume: "2"; pages: "e309" FEATURES Location/Qualifiers source 1..11454 /organism="Yersinia ruckeri" /plasmid="pYR1" /strain="YR71" /mol_type="genomic DNA" /db_xref="taxon:29486" mobile_element 1..11454 /mobile_element_type="transposon:Tn5393.11" /label=Tn5393.11 /note="Accession = Tn5393.11-CP000602.1; Family = Tn3; Group = Tn163; Synonyms =; Partial = ; Transposition = ; OtherInformation = ; Hosts: Organism = Yersinia ruckeri|Taxonomy = NCBI:txid29486|BacGroup = |MolecularSource = plasmid pYR1|Region = |Country = USA|OtherLocInfo = |DateIdentified = 2007|First = yes; Capture = yes " mobile_element 1..11454 /mobile_element_type="insertion sequence:IS1133::Tn10::IS903B" /label=IS1133::Tn10::IS903B /note="Accession = IS1133_Tn10_IS903B-CP000602.1; Family = IS3; Group =IS3; Synonyms = ; Partial = ; Transposition = ; OtherInformation = IS1133 (ISfinder:Z2167) with Tn10 insertion and IS903 insertion; Hosts: Organism =Erwinia amylovora |Taxonomy = |BacGroup = |MolecularSource = |Region = |Country = |OtherLocInfo = |DateIdentified = |First = ; Capture = yes" repeat_region 1..27 /label=IRL (IS1133::Tn10::IS903B) /note="Name = IRL; AssociatedElement = IS1133::Tn10::IS903B; LibraryName = IRL (IS1133); OtherInformation = ; Capture = yes" CDS 65..286 /codon_start=1 /transl_table=11 /gene="orfA_p" /locus_tag="YR71pYR1_0150" /product="N/A" /function="orfB-5'" /label=orfA_p (IS1133::Tn10::IS903B) /note="AssociatedElement = IS1133::Tn10::IS903B; Class = Accessory Gene; Subclass = Regulator; SequenceFamily = ; Chemistry = ; Target = ; LibraryName = orfA_p (IS1133_Tn10_IS903B); OtherInformation = orfA, together with orfB, encodes a fusion protein formed by translational frame-shifting that is the transposase for IS1133; Capture = yes " /db_xref="taxon:90371" /translation="MSLKHSDEFKRDAVRIALTSGLTRRQVASDLSIGLSTLGKWIASI SDETKIPTQDTDLLRENERLRKENGFVE" repeat_region complement(263..271) /locus_tag="YR71pYR1_0150" /label=DR misc_feature 264..271 /label=9 bp DR mobile_element 272..1328 /mobile_element_type="insertion sequence:IS903B" /label=IS903B /note="Accession = IS903B-CP000602.1; Family = IS5; Group = ; Synonyms =; Partial = ; Transposition = ; OtherInformation = 97% identical to reference sequence for IS903B (ISfinder:X02527); Hosts: Organism = |Taxonomy = |BacGroup = |MolecularSource = |Region = |Country = |OtherLocInfo = |DateIdentified = |First = yes; Capture = yes " repeat_region 272..289 /label=IRR (IS903B) /note="Name = IRR; AssociatedElement = IS903B; LibraryName = IR (IS903); OtherInformation = ; Capture = yes " CDS complement(328..1251) /codon_start=1 /gene="tnp" /product="Tnp" /function="transposase" /label=tnp (IS903B) /note="AssociatedElement = IS903B; Class = Transposase; Subclass = ; SequenceFamily = ; Chemistry = DDE; Target = ; LibraryName = tnp (IS903B); OtherInformation = ; Capture = yes" /translation="VAKQKFKITNWPTYNKALINRGSITFWLDDEAIQAWYESATPSSR GRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLRCPDYSCVSRRAKSVN VSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSKTHEIICA DLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHDELRRKKISALIPPRKGAGY WPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLRDYDGQ VAEAMALVRALNKMTKAGMPESVRIA" repeat_region complement(1311..1328) /label=IRL (IS903B) /note="Name = IRL; AssociatedElement = IS903B; LibraryName = IR (IS903); OtherInformation = ; Capture = yes " repeat_region complement(1329..1337) /label=DR misc_feature 1329..1337 /label=9 bp DR CDS 1466..2260 /codon_start=1 /gene="orfB_p" /product="N/A" /label=orfB_p (IS1133::Tn10::IS903B) /note="AssociatedElement = IS1133::Tn10::IS903B; LibraryName =orfB_p (IS1133_Tn10_IS903B); Class = Transposase; Subclass = ; Target = ; Chemistry = DDE; SequenceFamily = ; OtherInformation = Intact orfB, together with orfA, encodes a fusion protein formed by translational frame-shifting that is the transposase for IS1133; here, orfB is disrupted by insertion of Tn10; Capture = yes" /translation="MGVTDRGLRAWKRRPPSLRQRRDLILLAHIREQHRLCLGSYGRPR MTEELKALGLQVGQRRVGRLMRQNNITVVRTRKFKRTTDSHHTFNIAPNLLKQDFSASA PNQKWAGDITYVWTREGWVYLAVILDLYSRRVIGWATGDRLKQDLALRALNMALALRKP PPGCIQHTDRGSQYCAHEYQKLLLKHQLLPSMSGKGNCFDNSAVESFFKSLKAELIWRR HWQTRRDIEIAIFEYINGFYNPRRRHSTLGSDESPNDFGKNH" repeat_region complement(2214..2222) /gene="insD" /locus_tag="YR71pYR1_0152" /label=DR misc_feature 2214..2222 /label=9 bp DR mobile_element 2223..11369 /mobile_element_type="transposon:Tn10" /label=Tn10 /note="Accession = Tn10-AF162223; Family =compound; Group = IS10; Synonyms =; Partial = ; Transposition = ; OtherInformation = ; Hosts: Organism = Shigella flexneri|Taxonomy = NCBI:txid623|BacGroup = Enterobacteria|MolecularSource = plasmid R100|Region = |Country = U.S.A and U.K.|OtherLocInfo =|DateIdentified = 1975|First =; Capture = yes; " mobile_element 2223..3551 /mobile_element_type="insertion sequence:IS10R" /label=IS10R /note="Accession =IS10R-AF162223; Family = IS4; Group = IS10; Synonyms = ; Partial = ; Transposition = ; OtherInformation = 100% identical to IS10R (ISfinder:J01829); Capture = yes" repeat_region 2223..2239 /label=IRL (IS10R) /note="Name = IRL; AssociatedElement = IS10R; LibraryName = IRL (IS10); OtherInformation = ; Capture = yes;" CDS 2330..3538 /codon_start=1 /gene="tnp" /product="Tnp" /function="transposase" /label=tnp (IS10R) /note="AssociatedElement = IS10R; Class = Transposase; Subclass = ; SequenceFamily = ; Chemistry = DDE; Target = ; LibraryName = tnp (IS10R); OtherInformation = ; Capture = yes" /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQL VNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQ KQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYAL GKL" repeat_region complement(3530..3551) /label=IRR (IS10R) /note="Name = IRR; AssociatedElement = IS10R; LibraryName = IRR (IS10R); Capture = yes" CDS complement(3548..3964) /codon_start=1 /transl_table=11 /gene="tetD" /product="TetD" /label=tetD (Tn10) /note="AssociatedElement = Tn10; LibraryName = tetD (Tn10); Class = Passenger Gene; Subclass = Antibiotic Resistance; Target = tetracycline antibiotic (ARO:3000050); Chemistry = ; SequenceFamily = ; OtherInformation = no match in CARD; Capture = yes" /protein_id="AAD50249.1" /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQSL LLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQ FDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH" CDS 4052..4645 /codon_start=1 /transl_table=11 /gene="tetC" /locus_tag="YR71pYR1_0155" /product="TetC" /function="DNA binding (GO:0003677)" /label=tetC (Tn10) /note="AssociatedElement = Tn10; Class = Passenger Gene; Subclass = Antibiotic Resistance; SequenceFamily = Bacterial regulatory proteins, tetR family (Pfam:PF00440); Target = tetracycline antibiotic (ARO:3000050); Chemistry = ; LibraryName = tetC (Tn10); OtherInformation = no match in CARD; Capture = yes" /protein_id="ABO40921.1" /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKALV TKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNN KNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWF MHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK" CDS complement(4758..5963) /codon_start=1 /transl_table=11 /gene="tet(B) (ARO:3000166)" /product="Tet(B)" /function="antibiotic efflux (ARO:0010000)" /label=tet(B) (Tn10) /note="AssociatedElement = Tn10; Class = Passenger Gene; Subclass = Antibiotic Resistance; SequenceFamily = major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002); Target = tetracycline antibiotic (ARO:3000050); Chemistry = antibiotic efflux (ARO:0010000); LibraryName = tet(B) (Tn10); OtherInformation = strict match to reference sequence for ARO:3000166 (bitscore:786)||Synonym: tetB; Capture = yes" /protein_id="AAD50247.1" /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLG FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET SA" promoter 5971..6026 /gene="tetR" /label=tetR/tetA promoters /note="overlapping promoters for bacterial tetR and tetA" protein_bind 5972..5990 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 5972..5990 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 6002..6020 /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O1 for the tetR and tetA genes" protein_bind 6002..6020 /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O1 for the tetR and tetA genes" CDS 6042..6668 /codon_start=1 /gene="tetR (ARO:3003479)" /product="TetR" /function="antibiotic target alteration (ARO:0001001)||antibiotic efflux (ARO:0010000)" /label=tetR (Tn10) /note="AssociatedElement = Tn10; Class = Passenger Gene; Subclass = Antibiotic Resistance; SequenceFamily = major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002); Target = tetracycline antibiotic (ARO:3000050); Chemistry = repressor of the tetracycline resistance element; LibraryName = tetR (Tn10); OtherInformation = perfect match to reference sequence for ARO:3003479; Capture = yes" /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" CDS complement(6646..7332) /codon_start=1 /transl_table=11 /gene="jemC" /product="JemC" /label=jemC (Tn10) /note="AssociatedElement = Tn10; Class = Passenger Gene; Subclass = Other; SequenceFamily = HTH ArsR-type DNA-binding domain (InterPro:IPR001845); Target = ; Chemistry = ; LibraryName = jemC (Tn10); OtherInformation= N-terminal 100 amino acids similar to bacterial transcriptional repressors and metal-binding proteins (PMID:10781570); Capture = yes " /protein_id="AAD50245.1" /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATEL SSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHGVH AKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDV PSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAF KTHFHI" CDS complement(7340..7726) /codon_start=1 /gene="CPT07_26605" /locus_tag="CPT07_26605" /product="CPT07_26605" /label=CPT07_26605 (Tn10) /note="AssociatedElement = Tn10; Class = Passenger Gene; Subclass = Hypothetical; SequenceFamily = ; Target = ; Chemistry = ; LibraryName = CPT07_26605 (Tn10); OtherInformation = Amino acid-binding protein; Capture = yes; " /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAHF LVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYSDH ANQLILITDNDSMAASVTLPWAIK" CDS complement(7719..8039) /codon_start=1 /transl_table=11 /gene="jemB" /product="JemB" /label=jemB (Tn10) /note="AssociatedElement = Tn10; Class = Passenger Gene; Subclass = Other; SequenceFamily = Antibiotic biosynthesis monooxygenase (Pfam:PF03992); Target = ; Chemistry = ; LibraryName = jemB (Tn10); OtherInformation= ; Capture = yes; " /protein_id="AAD50244.1" /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSLA TEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKD NV" CDS 8483..9688 /codon_start=1 /gene="jemA" /product="JemA" /function="glutamate:sodium symporter activity (GO:0015501)" /label=jemA (Tn10) /note="AssociatedElement = Tn10; Class = Passenger Gene; Subclass = Other; SequenceFamily = ; Target = ; Chemistry = ; LibraryName = jemA (Tn10); OtherInformation= sodium-dependent glutamate permease; Capture = yes; " /translation="MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVA IVLLIIDKTSGYSFTFDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILI AIQNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMAC ATLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETIS MLIICIVVGGYISALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSV ALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVISA GHCGFGMGATPTAIANMQTVTKAFGPSHKAFLVVPMVGAFIVDISNSILIKIFIEIGTY FT" mobile_element 10041..11369 /mobile_element_type="insertion sequence:IS10L" /label=IS10L /note="Accession =IS10L-AF162223; Family = IS4; Group = IS10; Synonyms = ; Partial = ; Transposition = ; OtherInformation = 99% identical to IS10L (ISfinder:J01829); Capture = yes;" repeat_region 10041..10062 /label=IRR (IS10L) /note="Name = IRR; AssociatedElement = IS10L; LibraryName = IRR (IS10); Capture = yes" CDS complement(10054..11262) /codon_start=1 /gene="tnp" /product="Tnp" /function="transposase" /label=tnp (IS10L) /note="AssociatedElement = IS10L; Class = Transposase; Subclass = ; SequenceFamily = ; Chemistry = DDE; Target = ; LibraryName = tnp (IS10L); OtherInformation = ; Capture = yes;" /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIRTPKQL VNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQ KQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNLFTHGYVL GKL" repeat_region complement(11353..11369) /label=IRL (IS10L) /note="Name = IRL; AssociatedElement = IS10L; LibraryName = IRL (IS10); OtherInformation = ; Capture = yes;" repeat_region complement(11370..11378) /label=DR misc_feature 11370..11378 /label=9 bp DR repeat_region complement(11428..11454) /label=IRR (IS1133::Tn10::IS903B) /note="Name = IRR; AssociatedElement = IS1133::Tn10::IS903B; LibraryName = IRR (IS1133); OtherInformation=; Capture = yes" ORIGIN 1 tggtcttgcc tcggtttcgt tccggtcttt ttgagagcat tatttgttat caaggagacc 61 atatatgtca ttaaagcata gtgatgaatt taagcgtgat gcagttcgca tagcactcac 121 tagtggctta acacgccgtc aagttgcgtc agatttaagt attgggcttt ccacgcttgg 181 gaaatggatc gcatcaattt ccgatgaaac taaaattcct acccaagaca ctgatcttct 241 gcgtgagaat gaacgtttac gcaaagagaa cggctttgtt gaataaatca gatttcgggt 301 aagtctcccc cgtagcgggt tgtgttttca ggcaatacgc acgctttcag gcatacctgc 361 tttcgtcatt ttgttcagcg ctcgtaccag ggccatagcc tccgcaacct gaccatcgta 421 gtcacgcagc gtcagtgaac ccccgaacag ctgttttacc cggtacatcg ccgtttccgc 481 tatcgagcga cggttgtaat ctgttgtcca tttccaccgc gcattactcc cggtcattcg 541 ctgattagcc actgcacggt tacggtctgc atattcaccg ggccagtaac ccgcaccttt 601 tcggggaggg ataagcgcgc tgattttctt acgccgcagt tcatcgtgac atagccgggt 661 atcgtaagcg ccatcggcgg cggctgacct gattttccgg tgggtttgcc ggattaaccc 721 ggggaaggcc tctgagtccg taacgttgtt cagcgacagg tcagcgcaga tgatttcatg 781 tgttttactg tcaacggcga gatgcagctt acgccagata cggcggcgtt cctggccatg 841 ctttttgact ttccactcgc cttcaccgaa gaccttcagc ccggtggaat caattaccag 901 gtgtgcgatt tcaccccggg tgggcgtttt gaaactgaca ttaaccgact ttgcccgcct 961 gctgacacag ctgtaatccg ggcagcgtag cggaacgttc atcagagaaa aaatggaatc 1021 aataaagccc tgcgcagcgc gcagggtcag cctgaatacg cgtttaatga ccagcacagt 1081 cgtgatggca aggtcagaat agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc 1141 ataccaggcc tgaatagctt catcatccag ccagaaagtt atggagccac ggttgatgag 1201 ggctttattg taggtgggcc agttggtgat tttgaacttt tgctttgcca cggaacggtc 1261 tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca 1321 acaaagccaa agagaaccgt atccttcggg aggagaggga gatattaaaa aaggcagcaa 1381 tatttttcgc agtacaaaag ctgtgagatt tcagtttatt acggattacc gtggctctct 1441 ctcacgttca cgcatatgtc gtttgatggg cgtaacagat cgtggtttac gcgcatggaa 1501 acgccgtcct ccatcactgc gccagcgtcg tgatcttata cttctagcgc atatacgtga 1561 gcagcatcgg ttgtgtttgg ggagctatgg taggccgcgt atgacagaag agttgaaagc 1621 gctgggcctg caggttgggc agcgtcgggt tggacgtttg atgcgccaga ataacattac 1681 agttgttcga acgcgtaaat tcaaacggac aacggatagt catcatacct tcaacattgc 1741 accgaaccta ttaaaacaag actttagcgc aagcgcaccc aaccagaaat gggcaggcga 1801 tatcacttat gtttggacca gagaaggatg ggtctatctt gctgttatcc ttgacctgta 1861 ttcccgtcgc gtgattggct gggcaacagg tgatcgatta aagcaggatc ttgcattaag 1921 ggcactgaat atggcgttgg ctttacgcaa accaccaccg ggttgtattc aacacacaga 1981 ccgtgggagc caatattgcg ctcatgaata tcaaaagcta ctgctcaaac atcaattgct 2041 gccgtccatg agcgggaaag gcaattgttt tgataactcc gcagtagaaa gcttctttaa 2101 atcattaaag gctgagttga tttggcgcag acactggcaa acaaggcgag atattgagat 2161 tgcaatcttc gaatatataa atggctttta taatccacgc cgaagacatt caacactcgg 2221 ctctgatgaa tcccctaatg attttggtaa aaatcattaa gttaaggtgg atacacatct 2281 tgtcatatga tcaaatggtt tcgcgaaaaa tcaataatca gacaacaaga tgtgcgaact 2341 cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa 2401 cagcttaacg ttggcttgcc acgcattact tgactgtaaa actctcactc ttaccgaact 2461 tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt 2521 gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt 2581 tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata ttcgtgagca 2641 aaaacgactt atggtattgc gagcttcagt cgcactacac ggtcgttctg ttactcttta 2701 tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc aatttctagc 2761 cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg atgctggctt 2821 taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa gtcgagtaag 2881 aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca gcaacttaca 2941 tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta aaagcaatcc 3001 aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa atcagcgctc 3061 gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg caaaggagcc 3121 atgggttcta gcaactaact tacctgttga aattcgaaca cccaaacaac ttgttaatat 3181 ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg 3241 actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca tgctgctaat 3301 cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga aacaaggttg 3361 ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa cagttcgctt 3421 aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact tactcgtggc 3481 tgcaacccta ctagctcaaa atttattcac acatggttac gctttgggga aattatgagg 3541 ggatctctca gtgcattgcc tccaattccc ataatttatt acgccgataa taacttggtg 3601 taaccttaaa aatgtactta aatcgacgtg taaaagattg ttgggaatca aattgatatt 3661 ttaatgcgat ctcaaggata gtttttttcg tcaacctcaa ctcaacagcc gctttcgtca 3721 aacgacgagc acgaatatag ctagccagtg tgacccctgt tacttttttg aacagccgct 3781 gaaaatacca cttggtataa cccgctttat tcgccacatc atcaagcagt aaagactgat 3841 ctaaattatg ttcaatccat aatagaacat ctttgataac cgttgtatga aactgcttat 3901 catcatatct taattggatg ttattagctt tattttgata gcgagaatgc tgttcaatat 3961 acataaaata acctaaatgt tcttaagatt gtcacgacca catcatcatg ataccataaa 4021 catactgacg gtatgttatt ttaaatctat catggaaaat aaaaatcatc aacaagaaaa 4081 ttttaagagt acctatcaat cactggttaa ctcagcacga atattgtttg ttgaaaaagg 4141 ctatcaagct gtttcaatag atgagatctc gggaaaagcg ttggtgacca aaggtgcctt 4201 ttatcatcac tttaaaaata aaaaacaatt actcagtgcc tgttataagc agcaattaat 4261 tatgattgat gcctacatca caacaaaaac tgatttaaca aatggttggt ctgccttaga 4321 aagtatattt gaacattatc ttgattatat tattgataat aataaaaacc ttatccctat 4381 ccaagaagtg atgcctatca ttggttggaa tgaacttgaa aaaattagcc ttgaatacat 4441 tactggtaag gtaaacgcca ttgtcagcaa attgatccaa gagaaccaac ttaaagctta 4501 tgatgatgat gtgcttaaaa acttactcaa tggctggttt atgcatatcg caatacatgc 4561 gaaaaaccta aaagagcttg ccgataaaaa aggccaattt attgctattt accgcggctt 4621 tttattgagc ttgaaagata aataaaatag ataggtttta tttgaagcta aatcttcttt 4681 atcgtaaaaa atgccctctt gggttatcaa gagggtcatt atatttcgcg gaataacatc 4741 atttggtgac gaaataacta agcacttgtc tcctgtttac tcccctgagc ttgaggggtt 4801 aacatgaagg tcatcgatag caggataata atacagtaaa acgctaaacc aataatccaa 4861 atccagccat cccaaattgg tagtgaatga ttataaataa cagcaaacag taatgggcca 4921 ataacaccgg ttgcattggt aaggctcacc aataatccct gtaaagcacc ttgctgatga 4981 ctctttgttt ggatagacat cactccctgt aatgcaggta aagcgatccc accaccagcc 5041 aataaaatta aaacagggaa aactaaccaa ccttcagata taaacgctaa aaaggcaaat 5101 gcactactat ctgcaataaa tccgagcagt actgccgttt tttcgcccca tttagtggct 5161 attcttcctg ccacaaaggc ttggaatact gagtgtaaaa gaccaagacc cgctaatgaa 5221 aagccaacca tcatgctatt ccatccaaaa cgattttcgg taaatagcac ccacaccgtt 5281 gcgggaattt ggcctatcaa ttgcgctgaa aaataaataa tcaacaaaat gggcatcgtt 5341 ttaaataaag tgatgtatac cgaattcgat tgcgtctcaa cccctacttc ggtatctgta 5401 ttatcacgtg tatttttggt ttcacggaac caaaacataa ccacaaggaa agtgacaata 5461 tttagcaacg cagcgataaa aaagggacta tgcggtgaaa tctctcctgc aaaaccacca 5521 ataataggcc ccgctattaa accaagccca aaacttgccc ctaaccaacc gaaccacttc 5581 acgcgttgag aagctgaggt ggtatcggca atgaccgatg ccgcgacagc cccagtagct 5641 cctgtgatcc ctgaaagcaa acggcctaaa tacagcatcc aaagcgcact tgaaaaagcc 5701 agcaataagt aatccagcga tgcgcctatt aatgacaaca acagcactgg gcgccgacca 5761 aatcggtcag acatttttcc aagccaagga gcaaagataa cctgcattaa cgcataaagt 5821 gcaagcaata cgccaaagtg gttagcgata tcttccgaag caataaattc acgtaataac 5881 gttggcaaga ctggcatgat aaggccaatc cccatggcat cgagtaacgt aattaccaat 5941 gcgatctttg tcgaactatt catttcactt ttctctatca ctgataggga gtggtaaaat 6001 aactctatca atgatagagt gtcaacaaaa attaggaatt aatgatgtct agattagata 6061 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 6121 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 6181 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 6241 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 6301 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 6361 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 6421 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 6481 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 6541 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 6601 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 6661 ggtcttaaaa gcagcataac ctttttccgt gatggtaact tcacggtaac caagatgtcg 6721 agttaaccac cctttagatt cataaagcga aaataatgcg gctccaacgt acccacctaa 6781 atggaaacgg cgttcactcc aatctaaaca cgcacaacag attttacgtg aatgtttgga 6841 aggaacgtca attcccattt catgaaaata ttgaatacca cttaatgtga tcattgaacc 6901 attttcagtg atccattgct gttgacaaag ggaatcatag atcttaacgg caacttcgcc 6961 agctaaatga tcatagcaag tacgtgcttt tcgtaaatgc actggcgtgg aaactttggc 7021 atgtacgcca tggtttaagg agatccccat catactttcc atcaattcag caatatcttt 7081 tcctgctagc cgaaaataac gatgcttgcc ttgagctact actgtgatta gctggcaatc 7141 taataattta gataaatgac tgctcgccgt tgaagctgat atattcgcca cagaacttag 7201 ctcagtggcc gtccaagctc gcccatccat caaagcactg agtattttaa ctcgtgaaat 7261 gtcagacata gccgccccta tcgcggctat tgaggactca aaggtaacct cttttcgtat 7321 taaattagcc atcgcaagtt cactttattg cccaagggag cgtaacagat gcagccatac 7381 tatcattgtc cgttattaat atcagttggt tagcatggtc actgtattgc actaaaatat 7441 taatgttatt ctccgccaat actcgtgcta tttcgccaag ttcccccggt ttttcctgtt 7501 ttaacttacg aattaatggt gtccggatcg caagtactaa cagtccagct tgctctagcg 7561 ctattttagc ttcctttcct tgttcaacaa gaaaatgagc atggcattca tcaccaaccg 7621 taaatatccc tcccccttcc aatccaatac ctttattacc taatgttttt cctagtaatg 7681 ctaattgtcc tatttgatta tctaaaacaa cgtgaacatc aaacattatc cttgtccttt 7741 ttaaatgaat attcgcgagt aatatgagca atactaattt tgtaaaaatc aaatattgac 7801 tctcgccctt cttgttgagc tttcgcatgt aaaacatgat ttttccattg cagaactgcg 7861 tattcgtttt cccaccaaga tagcgataac atttttcctt ctgtagctag actttgaaaa 7921 cgttcaattg aaataaaacc agctacatga cttaatagtg gtcttaactc ctcagctaaa 7981 gtcaaatagc gagtttgttg gtcgggttgt atttgcacct caaatattac agcaatcata 8041 tttttctccg ttattaatca acaaacagag taatccaccc gaaaattaac acttcgataa 8101 gcaacgaaat atagaaaggt catgctgctg ttttatgttg tatatcacac ttctttaaat 8161 tgctcggcct gcgcttatca tgttctcctt ctcttataat tttgtttatt atttatactt 8221 ttttattttt tgctatcacc gaaaatagtg cggatcccgc atggtattta ggtttaccca 8281 tcattaaata ccattgttgt tattttaatc tttttaatat ttcatgatta agatcacact 8341 aaaattatga ccctattaac taaaagttat ggatacccta ataaaactca ttgttaaaga 8401 ttagttaacc aaagtaactt catttttcac tattgttaaa ttgttatagt gaaaattctg 8461 gtaaactaat agaggtaaaa aaatgatcct agatgccagt tatacattat tagtcgcatg 8521 tatcgcgcta ctcataggaa tgtttgtcgt aaaatttacc ccgttcctac aaaaaaacca 8581 cataccagaa gccgtcgttg gtggctttat tgttgcaatt gttctgttaa ttattgataa 8641 aacatcaggt tattcgttta cttttgatgc ttcattgcaa agtttattaa tgctcacatt 8701 cttttcctct atcgggctaa gttctgactt ttctcgactg attaaaggag gaaagccgtt 8761 agttctatta actattgcag taacgatcct aatcgccatt caaaatactg tcggcatgag 8821 tatggctgtc atgatgaatg aaagtccatt tattggctta attgcaggtt caattactct 8881 aacaggtggt catggtaatg ccggagcatg gggccctatt ctcgctgata aatatggtgt 8941 aacaggcgcc gttgaattag cgatggcttg tgcaacactt ggattagtgt tgggtggttt 9001 agttggcggc cctgttgccc gtcatctttt gaaaaaggtc tctattccta aaacaaccga 9061 gcaagagcgc gacactatcg ttgaagcttt tgagcaacca agcgtcaaaa gaaaaatcaa 9121 tgcaaataac gttattgaaa ccatttcaat gctgattatc tgtattgttg ttggtggcta 9181 tatcagtgca ttgtttaaag atacttttct gcaactgcct acttttgtct ggtgtttatt 9241 tgtcggtatt attatccgta atacactgac tcatgtattt aaacacgaag tgtttgagcc 9301 gaccgtcgat gtattaggta gcgttgcttt atcgcttttc ttggcaatgg cgttaatgtc 9361 attaaaattt ggtcaattgg caagcatggc agggccagta ttaattatca ttgctgtaca 9421 aactgttgtc atggtgctat ttgcctgctt tgtcaccttc aaaatgatgg gcaaagatta 9481 tgatgctgtc gtgatcagcg cgggtcactg tggctttggt atgggagcaa caccaacagc 9541 aattgcgaat atgcaaacag tcacaaaagc atttggacca tcacataaag ctttccttgt 9601 cgttcctatg gtcggtgcct ttattgttga tatttcaaac agtatcttaa ttaaaatatt 9661 tattgaaatt ggtacatact ttacctaata gctacacact actcggcgta cttctcctaa 9721 caatttatta tagggcagta tgccgagccg ctttcttacc ccgtatattc ccctagtcaa 9781 tctatttagc ttgcacgttt acgaattaat tctcgtgaca actaaaccaa aatttgaatt 9841 atgacgagta tatctgatta tttatacttt attcgttaga atagtccgta gaaataaata 9901 atcagtaggc tacatggaat gaaattaaga catctcgata tcttttatgc tgtaatgact 9961 tgtggttcct taacgcgtgc ggctgaagtt ttacatattt ctcaacccgc ggcgagtaaa 10021 gcactcaaac atgctgagca ctgagagatc ccctcataat ttccccaaaa cgtaaccatg 10081 tgtgaataga ttttgagtaa gcagggttgc agccacgagt gagtcttccc ttgttattgt 10141 gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta cgtttcgatt 10201 tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat gaacgcccgc 10261 aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa aacgctctga 10321 gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca agtctcggaa 10381 ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg gtgttcgaat 10441 ttcaacaggt aagttagttg ctagaatcca tggctccttt gccgacgctg agtagatttt 10501 aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc ctttagagcg 10561 agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc tcttatagcc 10621 taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt tccagttttc 10681 cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt accaacccag 10741 cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa tgagcggtgt 10801 ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag ctttctttga 10861 acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac cgtgtagtgc 10921 gactgaagct cgcaatacca taagccgttt ttgctcacgg atatcagacc agtcaacaag 10981 tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt atacagcgag 11041 tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga tgttatgttt 11101 tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag ttttacagtc 11161 aagtaaggcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta attcggggca 11221 gaattggtaa agagagtcgt gtaaaatatc gagttcgcac attttgttgt ctgattattg 11281 atttttggcg aaaccatttg atcatatgac aagatgtgta tctaccttaa cttaatgatt 11341 ttgataaaaa tcattagggg attcatcagc actcggctgg aaatcgccgg tggcatttga 11401 gaaaaaagcc gcttaaaatg agagatagac cggaacacaa ccggtgcaag acca //